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MAKALAH BEBAS Antimalarial ... Penyakit Demam Berdarah Dengue di Kabupaten Bulukumba, ... Analisis Porsi Substansi V ektor Malaria dalam Perencanaan...

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Dalam Rangba Hari

PROSIDING SEMINAR NASIONAL HARI NYAMUK 2009 "Partisipasi Masyarakat dalam Program Pengendalian Nyamuk Terpadu"

Dolam Rangba Hari Nyamul:l Nasional2009 IPB International Convention Center - Botani Square Bogor Senin, 1() Agustus 2009

EDITOR: Upik Kesumawati Hadi Sri Utami Handajani . ~luna Risa T. Susi Soviana

ISBN 978-602·95733-0-5 PENYELENGGARA

Sekretariat : Bagian Parasitologi dan Entomologi Kesehatan, FKH - IPB

Jl. Agatis Kampus IPB Darmaga Bogor, Telp./Fax. 02518421784 Email : [email protected], [email protected]

PROSIDING SEMINAR NASIONAL HARI NYAMUK 2009 "Partisipasi Masyarakat dalam Program Pengendalian Nyamuk Terpadu"

Datam .RaP..gka Hati Nyamuk Nasional2009

ISBN 978-602·95733-0-5 @2009

Hak Cipta dilindungi Undaag-unda.r.g Diterbitkan oleh Bagian Parasitologi dan Entomologi Kesehatan Faktdtas Kedokteran Hewan Institut Pertanian Bogor JI. Agatis Kampus IPB Darmaga Bogor Telp/Fax. 0251 8421784 Email : [email protected]

Desain cover dan layout : Sugiarto

DAFTARISI KATA PENGANTAR ...............................................................................

1

DAFTAR ISI .................................................................................................

u

SAMBUTAN KETUA PANITIA.............................................................

1v

SAMBUTAN REKTOR IPB......................................................................

Vl

KEYNOTE SPEAKER Progra..
1

PLENNARY SESION ................................................................................

13

Konsep Pengendalian Hama Terpadu.........................................................

13

Penelitian Resistensi Vektor di Indonesia (Biomolekuler) .......................

23

Pemberdayaan Masyarakat untuk Mengendalikan Vektor (COMB!

Approach) ...........................................................................................................

34

Pengalaman Penerap:m Peraturan Daerah (Law Enforcement) dalam Pengendalian DBD di DKI Jakarta.............. ;....................,..........................

44

New Technology for Mosquitoes Control... .............................................. .

54

v

MAKALAH BEBAS Antimalarial Compounds from Erythrina variegata (Leguminosae) ......... .

58

Analisis Faktor Faktor Densitas Larva Aedes aegypti dan Endemisitas Penyakit Demam Berdarah Dengue di Kabupaten Bulukumba, Sulawesi Selatan .....................................;........................................................ .

67

Pengaruh Komposisi Media Fermentasi Metarhitfum sp. terhadap Mortalitas Larva Aedes aegypti........................................................................ .

75

Analisis Porsi Substansi Vektor Malaria dalam Perencanaan Pembangunan Keseha.tr.n Tahun 2007-2010 di Kabupaten Kupang Provinsi Nusa Tenggara Timur.................................................................... .

85

Perilaku Bertelur NyamukAedes aegypti pada Air Sumur Gali dan Air Comberan ........................................................................................................ .

92

..

Penyelenggara

11

Sebaran Tambak Benur dan Risikonya sebagai Habitat Larva Anopheles ........................................................................................................ .

99

Dayatetas Telur Aedes aegypti pada Air Tercemar ...................................... .

107

Populasi Nyamuk Tersangka Vektor Filariasis di Daerah Endemis Desa Jambu Ilir Kabupaten Ogan Komering Ilir Sumatera Selatan ...... .

120

K...andungan Kimia Bawang Putih sebagai Rcpelen Nyanmk. .................. .

129

Fengendaliail Penyakit DBD der.gan Tt:knik Sera!lgga Mandul (fSM.).

137

Habitat]entikAedes aegypti (Diptera: Culicidae) pada Air Terpolusi di Laboratorium .................................................................................................. .

143

v

Sebaran jentik NyamukAedes aegypti (Diptera: Culicidae) di Desa Cikarawang, Kabupaten Bogar .................................................................... .

154

v

Pemetaan Tempat Perindukan Larva NyamukAnopheles sp. di Kayangan-Lombok Utara, Nusa Tenggara Barat..................................... .

160

LAMP IRAN

Daftar Panitia Penyelenggara.................. ... .......... ..... ...... ....... ... ........ ..

167

Daftar Peserta Seminar........................................................................

169

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a/

Study of the effect of ultrasonic device against the dengue mosquito, Aedes aegypti (Diptera: Culicidae) Upik Kesumawati Hadi, FX. Koesharto, SH Sigit &Sugiarto Div. Parasitology and Medical Entomology Fac. Vet. Med. Bogor Agricultural University, Indonesia Jl Agatis, Dannaga Campus, Bogor 16680 Telp/Fax 0251 8421784, email [email protected] ABSTRACT - The study of the effect of ultrasonic device against the dengue mosquito, Aedes aegypti adults was done in Peet Grady chamber. Three replications of tests were performed based on 24 hours expositions. 50 female mosquitoes on each replication were introduced by ultrasonic device (type ICE) in Peet Grady Chamber, and the other 50 females were put in other Peet Grady Chamber without the device. Observation and counts on !mock down mosquitos (mortality rate) \Yere done every one hour to six hours, then 24 hour after exposure. The result showed that During the first hour observation, 2.67% of mosquitoes were knockdown (died) by ultrasonic device (ICE typej. In the long run (one to six hours) observation, 3.33 to 10% of the mosquitoes were died, however, after 24 hours exposure 74% of the mosquitoes died. On the control groups, all of the mosquitoes (100%) were still alive on the six hour observations, and after 24 hour observations only 7.33% mosquioes died. Based on the observation, the ultrasonic device (ICE type) affected the mosquitoes after 24 hours observations by gMng them knock down efect and finally died (74%). KEY WORD: Aedes aegypti, knockdown effect mosquito, ultrasonic.

INTRODUCTION The recent increase in dengue epidemics all around the world and the real risk· of resurgence of urban yellow fever has directed public concern on how to avoid Aedes aegypti (l.) and Aedes albopictus (Skuse) bites. The avoidance of mosquito bites consequently turns to the obvious- personal protection and how to obtain the perfect . repellent or other devices without insecticides. Sonic, electronic and ultrasonic devices have all been advertised as repelling mosquitoes. Some devices are available in a

bracelet, watch or neckloce fonn for personal protection while others plug into room outlets and claim to eliminate mosquitoes from a larger area (Banido et at. 1993). Ultrasound Is a fonn of cyclic sound pressure with a frequency greate~ than the upper limit of human hearing, this limit being approximately 20 kilohertz (20,000 hertz). The upper frequency limit in humans {approximately 20 kHz) is caused by the middle ear, which a;ts as a low-pass filter. If ultrasound is fed directly Into the skull bOne and reaches the cochlea without passing through the middle ear, much higher frequencies can be heard. This effect (sometimes called ultrawnic hearing) was first discovered by divers exposed ttl high frequencies (ca 50 kHz) sonar signal. Carefully-designed scientific studies have been performed and confirmed what they call the hyperso11ir. effect - that aven without consciously heaiing it, high-frequency sound can have a mcasurabie effect on tne mind. It is a standarc! fact in psychoacoustics that children can haar some highpitched sounds that older adults cannot hear, becau&e in humans the upper limit pitch of hearing tends to become lower with age (Takeda eta/. 1992). However, in Indonesia there is no information about the effect of the ultrasonic davices against the mosquitoes. Here, the research was aimed to study the effect of ultrasonic devices against the dengue mosquito, Aedes aegypti.

MATERIAL ANU METHODS Product tested was ultrasonic device (ICE type), 20kHz - 100kHz, 12 volt, 12 minutes interval prodw..e by LG. The test was carried out in Entomology Laboratory, Division of Parasitology and Medical Entomology, Department of Animal Diseases and Veterinary Public Health, Fa:ulty of Veterinary Medicine, Boger Agricultural Unicersity, Bogar Indonesia. The test was done from February to April 2009. The mosquito used in this study was Aedes aegypti, strain livP.rpool, female, 4-5 days old, colonized in Entomology laboratory. The test were done in 190x190x190 cm3 Peet Grady (PG) chambers. Four to five days old Aedes agypti were use for the test Ultrasonic device (ICE type) was put inside rignt at the center or the PG and used, waited for one minute. Fivety sucrose-fed females were released into PG chamber, observed for several minutes to make sure that no mosquito fell down to the floor. Observation and counts on knock down mosquitos were done every hour up to six hours, then 24 hours after exposure. RESULTS AND DISCUSSION The result of the research was performed in Table 1. The result showed that during the first hour observation, 2.67% of mosquitoes were knockdown {died) by ultrasonic device (ICE type). In the tong run (one to six hours) observation, 3.33 to 10% of the mosquitoes were died, however, after 24 hours exposure 74% of the mosquitoes died. On the control groups, all of the mosquitoes (100010) were still alive on the six hour observations, and after 24 hour observations only 7.33% mosquioes died.

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Based on the observation, the ultrasonic device (ICE type) affected mosquitoes after 24 hours observations by giving them knock down effect and finally died (74%). According to th6 theory is that rodents and iilsects can hear sounds that are above the range of human senses (upper than 20 kHz). By emitting a loud ultrasound, it's assumed that the mosquitoes will received the effect of ultrasonic wave through their antennae as receptors, and their wiil be repeiled, driven Insane or discomfort and finally dead. Table 1. Mortaliiy rate of mosquioes after exposured by ultrasonic device (ICE type, 20-100 kHz, 12 volt 12 minutes int9rval) in Peet Grady Chamber

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2 3 4 5 6 24

. 4.00 6.00 12.00 12.00 14.00 20.00 82.00

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0.00 0.00 0.00 0.00 0.00 2.00 56.00

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4.00 4.00 4.00 4.00 6.00 8.00 84.00

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2.67 3.33 5.33 5.33 6.67 10.00 74.00

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0.00 0.00 0.00 0.00 0.00 0.00 6.00

0.00 0.00 0.00 0.00 0.00 0.00 8.00

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O:QQ_ 0.00 0.00 0.00 0.00 0.00 7.33

CONCLUTION Ultrasonic device (ICE type, 20 kHz - 100 kHz, 12 volt, 12 minute intervaQ affected the mosquitoes (Aedes aegyptJ) after 24 hours observations by giving them (74%) knock down effect and finally died. REFERENCES

Barrido R., Brown J., Novak R. and Berenbaurn B. 1993. A test of the efficacy of ultrasonic mosquito repellers. The Vector Control Bulletin of the North Central States.

2:65-69. Takeda Set 81. (1992) Age variation in the upper limit of hearing. European Journal of Applied Physiology 65(5), 403-408.

Diskusi: 1. Penanya pertamc. a. Diketahui kontrol hanya 6 jam masa pengujiannya sedangkan yang menggunakan ultrasound hingga 24 jam, mengapa kontrolnya juga tidak diuji sama dengan pat1akuan yang menggunakan ultrasound yattu 24 jam? b. Pada kesimpulan tidak mencantumkan efektifttas? Jawab:

a. Kelompok kontrol dan perlakuan dengan ultrasound diamati dalam waktu yang sama yaitu 24 jam, sedangkan untuk efektifrtas dari kelompok dengan perlaku~n adalah 74% SP.dangkan u nt•Jk kontrol hanyel 7.33% dalam waktu 24jam. b. Penelitian entoroologi berkcr.an dengan masalah efikasi alat, sedangkan untuk keamanan digunakan penelitian terhadap monyet yang memiliki fungsi fisiologis hamper menyerupai manusia. Pengaruh gelombang ultrasound dalam jangka panjang dengan FK UNPAD

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